i was at the lab for a little while yesterday and today because i have been working on some cloning that is just not going well. i have repeated this step about 5 times over the past 2 weeks, and haven't been having any success. FINALLY when i went in and looked at the plates i had prepared on friday night, i had a bunch of colonies meaning that my ligation (probably) worked. i'll be (hopefully) confirming that tomorrow, but i finally have some hope that i will soon be done with this cassette and can move on. small victory.
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